Modification of a single-molecule AFM probe with highly defined surface functionality

• Fei Long,
• Bin Cao,
• Ashok Khanal,
• Shiyue Fang and
• Reza Shahbazian-Yassar

Beilstein J. Nanotechnol. 2014, 5, 2122–2128, doi:10.3762/bjnano.5.221

• area would result in a highly defined surface functionality of the probe down to single molecule level with high reproducibility. Keywords: atomic force microscopy; click reaction; force spectroscopy; single molecule modification; Introduction Single-molecule force spectroscopy (SMFS) has become one

• resulting larger contact area between probe and surface during force spectroscopy. The value of nav of the specific interaction was 1.1–1.5 for the Si probe, demonstrating a successful single-molecule modification. Similar values of nav were obtained for the other three Si probes with a maximum value of 2.1